Plasmid

Part:BBa_K4761429:Design

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-09-30)


pCas


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6256
    Illegal EcoRI site found at 10357
    Illegal EcoRI site found at 11866
    Illegal XbaI site found at 9054
    Illegal SpeI site found at 2715
    Illegal PstI site found at 4500
    Illegal PstI site found at 11361
    Illegal PstI site found at 11608
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6256
    Illegal EcoRI site found at 10357
    Illegal EcoRI site found at 11866
    Illegal NheI site found at 6015
    Illegal SpeI site found at 2715
    Illegal PstI site found at 4500
    Illegal PstI site found at 11361
    Illegal PstI site found at 11608
    Illegal NotI site found at 3034
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6256
    Illegal EcoRI site found at 10357
    Illegal EcoRI site found at 11866
    Illegal BamHI site found at 8294
    Illegal BamHI site found at 10296
    Illegal XhoI site found at 4353
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6256
    Illegal EcoRI site found at 10357
    Illegal EcoRI site found at 11866
    Illegal XbaI site found at 9054
    Illegal SpeI site found at 2715
    Illegal PstI site found at 4500
    Illegal PstI site found at 11361
    Illegal PstI site found at 11608
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6256
    Illegal EcoRI site found at 10357
    Illegal EcoRI site found at 11866
    Illegal XbaI site found at 9054
    Illegal SpeI site found at 2715
    Illegal PstI site found at 4500
    Illegal PstI site found at 11361
    Illegal PstI site found at 11608
    Illegal AgeI site found at 10131
    Illegal AgeI site found at 11788
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 10113


Design Notes

Source

References